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Applied Biological Materials Inc all in one rt mastermix
All In One Rt Mastermix, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/all+in+one+rt+mastermix/all+in+mastermix+one+rt/pmc13280338-146-16-19
Average 86 stars, based on 1 article reviews
all in one rt mastermix - by Bioz Stars, 2026-10
86/100 stars

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Synthesized:

Article Title: Semaglutide-induced loss of skeletal muscle mass is blunted by co-administration of ketone esters.
Article Snippet: 666 667 Quantitative RT-PCR: 668 669 Total RNA was extracted from frozen tissue samples using TRIzol reagent (Invitrogen, Carlsbad, 670 CA), following the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized 671 using 5× All-In-One RT MasterMix (Cat# G486, Applied Biological Materials, Vancouver, BC), 672 also according to the manufacturer’s protocol. .. Quantitative real-time PCR was performed using 673 PowerUp SYBR Green Master Mix (Cat# A25742, Applied Biosystems, Foster City, CA) in white 674 384-well plates on a LightCycler 480 system (Roche Life Science), as previously described (45).

Article Title: Methoprene and dsSepbanr co-delivered RNAi nano-system: a dual-mechanism strategy for targeted disruption of development and reproduction in Spodoptera exigua.
Article Snippet: Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae), is a significant agricultural pest worldwide.. Juvenile hormone (JH) plays a crucial regulatory role in insect mating behavior and sex pheromone synthesis.. In this study, pH-responsive nanocarriers (methoprene/UiO-66-NH2 and dsSepbanr/UiO-66-NH2@PEI) were developed to investigate the synergistic insecticidal effect of the juvenile hormone analog (methoprene) and dsRNA targeting the Sepbanr gene in Spodoptera exigua.

Article Title: B1 cells drive pulmonary emphysema progression through ADAM10
Article Snippet: .. Briefly, total RNA was isolated using TRIzol reagent (Cat. No. 15596018; Thermo Fisher Scientific), and cDNA was synthesized using the 5× All-In-One RT MasterMix (Applied Biological Materials, Richmond, BC, Canada). qPCR was performed on an ABI Prism 7500 Sequence Detection System (Thermo Fisher Scientific) using BlasTaq 2× qPCR MasterMix (Cat. No. G891; Applied Biological Materials), according to the manufacturer’s protocol. ..

Reverse Transcription:

Article Title: Hybrid membrane nanovesicles reprogram macrophages to resolve oxidative stress in intervertebral disc degeneration
Article Snippet: .. Subsequently, 1 μg of total RNA was reverse transcribed into cDNA using a commercial 5 × All-In-One RT MasterMix (Applied Biological Materials). .. Quantitative real-time PCR (qRT-PCR) was performed using SYBR Green qPCR Master Mix (Vazyme) on a real-time PCR detection system.

Article Title: Potential Activity of 6-Pentyl-α-pyrone as an Antiviral for Bovine Coronavirus
Article Snippet: .. To assess viral NP gene expression, MDBK cells were infected with BCoV at MOI 0.5 and treated or not with 6PP for 48 h. Then, total RNA was extracted by Trizol reagent (Thermo Fisher, Waltham, MA, USA) and reverse transcribed into cDNA with an All-In-One RT MasterMix (Applied Biological Materials, Richmond, BC, Canada). .. The cDNA was amplified via real-time PCR using a BlasTaq 2 qPCR mastermix (Applied Biological Materials, Richmond, BC, Canada) and 0.1 μM primers from Eurofins.

Article Title: Potential Activity of 6-Pentyl-α-pyrone as an Antiviral for Bovine Coronavirus
Article Snippet: .. To assess viral NP gene expression, MDBK cells were infected with BCoV at MOI 0.5 and treated or not with 6PP for 48 h. Then, total RNA was extracted by Trizol reagent (Thermo Fisher, Waltham, MA, USA) and reverse transcribed into cDNA with an All-In-One RT MasterMix (Applied Biological Materials, Richmond, BC, Canada). .. The cDNA was amplified via real-time PCR using a BlasTaq 2 qPCR mastermix (Applied Biological Materials, Richmond, BC, Canada) and 0.1 μM primers from Eurofins.

Article Title: Transcriptomics and metabolomics provide insights into meat flavor precursor differences between different duck lines
Article Snippet: The quality and purity of the RNA were assessed with a Nanodrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE). .. Following DNase treatment, 1 μg of total RNA from each sample was reverse-transcribed into cDNA using the 5X All-In-One RT MasterMix (Applied Biological Materials Inc., Richmond, BC, Canada). ..

Gene Expression:

Article Title: Potential Activity of 6-Pentyl-α-pyrone as an Antiviral for Bovine Coronavirus
Article Snippet: .. To assess viral NP gene expression, MDBK cells were infected with BCoV at MOI 0.5 and treated or not with 6PP for 48 h. Then, total RNA was extracted by Trizol reagent (Thermo Fisher, Waltham, MA, USA) and reverse transcribed into cDNA with an All-In-One RT MasterMix (Applied Biological Materials, Richmond, BC, Canada). .. The cDNA was amplified via real-time PCR using a BlasTaq 2 qPCR mastermix (Applied Biological Materials, Richmond, BC, Canada) and 0.1 μM primers from Eurofins.

Article Title: Potential Activity of 6-Pentyl-α-pyrone as an Antiviral for Bovine Coronavirus
Article Snippet: .. To assess viral NP gene expression, MDBK cells were infected with BCoV at MOI 0.5 and treated or not with 6PP for 48 h. Then, total RNA was extracted by Trizol reagent (Thermo Fisher, Waltham, MA, USA) and reverse transcribed into cDNA with an All-In-One RT MasterMix (Applied Biological Materials, Richmond, BC, Canada). .. The cDNA was amplified via real-time PCR using a BlasTaq 2 qPCR mastermix (Applied Biological Materials, Richmond, BC, Canada) and 0.1 μM primers from Eurofins.

Infection:

Article Title: Potential Activity of 6-Pentyl-α-pyrone as an Antiviral for Bovine Coronavirus
Article Snippet: .. To assess viral NP gene expression, MDBK cells were infected with BCoV at MOI 0.5 and treated or not with 6PP for 48 h. Then, total RNA was extracted by Trizol reagent (Thermo Fisher, Waltham, MA, USA) and reverse transcribed into cDNA with an All-In-One RT MasterMix (Applied Biological Materials, Richmond, BC, Canada). .. The cDNA was amplified via real-time PCR using a BlasTaq 2 qPCR mastermix (Applied Biological Materials, Richmond, BC, Canada) and 0.1 μM primers from Eurofins.

Article Title: Potential Activity of 6-Pentyl-α-pyrone as an Antiviral for Bovine Coronavirus
Article Snippet: .. To assess viral NP gene expression, MDBK cells were infected with BCoV at MOI 0.5 and treated or not with 6PP for 48 h. Then, total RNA was extracted by Trizol reagent (Thermo Fisher, Waltham, MA, USA) and reverse transcribed into cDNA with an All-In-One RT MasterMix (Applied Biological Materials, Richmond, BC, Canada). .. The cDNA was amplified via real-time PCR using a BlasTaq 2 qPCR mastermix (Applied Biological Materials, Richmond, BC, Canada) and 0.1 μM primers from Eurofins.

Isolation:

Article Title: B1 cells drive pulmonary emphysema progression through ADAM10
Article Snippet: .. Briefly, total RNA was isolated using TRIzol reagent (Cat. No. 15596018; Thermo Fisher Scientific), and cDNA was synthesized using the 5× All-In-One RT MasterMix (Applied Biological Materials, Richmond, BC, Canada). qPCR was performed on an ABI Prism 7500 Sequence Detection System (Thermo Fisher Scientific) using BlasTaq 2× qPCR MasterMix (Cat. No. G891; Applied Biological Materials), according to the manufacturer’s protocol. ..

Real-time Polymerase Chain Reaction:

Article Title: B1 cells drive pulmonary emphysema progression through ADAM10
Article Snippet: .. Briefly, total RNA was isolated using TRIzol reagent (Cat. No. 15596018; Thermo Fisher Scientific), and cDNA was synthesized using the 5× All-In-One RT MasterMix (Applied Biological Materials, Richmond, BC, Canada). qPCR was performed on an ABI Prism 7500 Sequence Detection System (Thermo Fisher Scientific) using BlasTaq 2× qPCR MasterMix (Cat. No. G891; Applied Biological Materials), according to the manufacturer’s protocol. ..

Sequencing:

Article Title: B1 cells drive pulmonary emphysema progression through ADAM10
Article Snippet: .. Briefly, total RNA was isolated using TRIzol reagent (Cat. No. 15596018; Thermo Fisher Scientific), and cDNA was synthesized using the 5× All-In-One RT MasterMix (Applied Biological Materials, Richmond, BC, Canada). qPCR was performed on an ABI Prism 7500 Sequence Detection System (Thermo Fisher Scientific) using BlasTaq 2× qPCR MasterMix (Cat. No. G891; Applied Biological Materials), according to the manufacturer’s protocol. ..



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